1. Isingeniso
I-viral nucleic acid extraction kit ilungele ukukhishwa okuphelele kwe-nucleic acid kusuka ku-serum, i-plasma, i-homogenate yezicubu namanye amasampula. I-kit isuselwa kubuchwepheshe be-Silica Clology Curication Technology, eqeda isidingo sokukhishwa okunobuthi obunobuthi kanye nokudla isikhathi sotshwala isikhathi. Lo mkhiqizo ukhiphe ngempumelelo ama-nucleic acid kusuka ku-hepatitis ba / c, hepatitis C, ne-norovirus standard. I-DNA / RNA etholakele ingasetshenziswa ngokuqondile ochungechungeni lwe-Downstream Presstream efana ne-PCR, RT-PCR, nesibani.
Qaphela:
1. I-Carrier RNA Solid kumele ichithwe emanzini angenamanzi we-nuclease kuya ku-1 ec / μl ngaphambi kokusetshenziswa, kanye ne-vortex ukuncibilikisa. Gcina kuma-aliquots ku -70 ° C. Uma udinga ukuyigcina ku-20 ℃ isikhathi eside, sicela uyibuyise ngokuya ngenani lezikhathi zokusetshenziswa.
2. Dissolve Proteinase K (20mg / ML): Faka amaprotheni ancibilikisa i-buffer ukuncibilikisa amaprotheni bese ukugcwaliswa kokugcina kwe-20mg / ml. I-protenase k i-powder eyomile ingagcinwa ku-2-8 ° C unyaka owodwa, kepha iprotheni ecijile k kufanele igcinwe kuma-aliquots at -20 ° C. Ukuphindaphindwa okuphindaphindiwe nokuncipha kweprotheni K kungathinta umsebenzi wayo.
3. I-Buffer VHB kumele ihlanjululwe nge-14 ml ngokuphelele ethanol ngaphambi kokusebenzisa futhi igcinwe emazingeni okushisa asekamelweni.
4. I-Buffer RW2 kufanele ihlanjululwe nge-80 ml ye-ethanol ephelele ngaphambi kokusetshenziswa futhi igcinwe emazingeni okushisa asekamelweni.
3. Impilo yeshelufu
Ngaphandle kweProteinase K kanye ne-Carrier RNA, ezinye izakhi zalo mkhiqizo zingagcinwa emazingeni okushisa asekamelweni (15-25 ° C) izinyanga eziyi-12, futhi kufanele zigcinwe ku-2-8 ° C ukuze isitoreji isikhathi eside. I-Proteinase K ne-Carrier RNA eyomile i-powder ihanjiswa emazingeni okushisa asekamelweni. Sicela ugcine ku-At -20 ° C ngemuva kokuthola umkhiqizo wokuhlola, bese ugcina ku-20 ° C ngemuva kokuncibilika.
